Despite its many advantages, GFP has some limitations:
Phototoxicity: Prolonged exposure to the excitation light needed for GFP fluorescence can cause damage to cells, potentially affecting the results. Bleaching: GFP fluorescence can fade over time, a phenomenon known as photobleaching, which may limit long-term studies. Immune Response: In some cases, the introduction of GFP into an organism can elicit an immune response, which can complicate experimental outcomes.
