3D models differ from 2D cultures in several ways:
Spatial Organization: 3D models maintain the spatial organization of cells, similar to in vivo conditions, whereas 2D cultures are flat and lack this complexity. Cell-Cell Interactions: They allow for more realistic cell-cell and cell-matrix interactions, which are critical for studying cancer behavior. Drug Penetration: 3D models better replicate the challenges of drug penetration and distribution seen in real tumors.
