The creation of CD34 humanized mice involves several steps: 1. Immunodeficient Mice: Researchers start with immunodeficient mice, such as the NOD/SCID or NSG strains, which lack functional T cells, B cells, and NK cells. 2. Human CD34+ Cell Isolation: Human HSCs are isolated from sources such as umbilical cord blood, bone marrow, or peripheral blood. 3. Engraftment: The isolated CD34+ cells are then introduced into the immunodeficient mice through intravenous injection or intrahepatic injection in neonatal mice.
