How do Western Blotting and ELISA contribute to cancer research?
Western Blotting and ELISA (Enzyme-Linked Immunosorbent Assay) are techniques used to detect and quantify proteins. Western blotting separates proteins by electrophoresis, followed by transfer to a membrane and detection using specific antibodies. ELISA quantifies proteins in a sample by binding them to specific antibodies and measuring the enzymatic reaction. These techniques are essential for investigating protein expression, post-translational modifications, and signaling pathways in cancer cells.
