The process of amplicon sequencing involves several steps:
Primer Design: Specific primers are designed to target regions of interest in the genome. DNA Extraction: DNA is extracted from cancer tissues or liquid biopsies. PCR Amplification: The target regions are amplified using PCR, generating multiple copies of the DNA fragments. Sequencing: The amplified fragments are sequenced using high-throughput sequencing technologies. Data Analysis: The sequencing data is analyzed to identify genetic variations and their potential significance in cancer.
