The process of RNA-Seq typically involves several key steps. First, RNA is extracted from the sample, followed by the selection of mRNA or total RNA. The RNA is then converted into complementary DNA (cDNA) using reverse transcription. Subsequently, the cDNA is fragmented and sequenced using high-throughput sequencing technologies. The resulting sequences are mapped to a reference genome, and data analysis is performed to quantify gene expression levels and identify novel transcripts.
