Single cell proteomics involves isolating individual cells from a tumor and then analyzing their protein content using advanced techniques such as mass spectrometry or antibody-based methods. Technologies like CyTOF (Cytometry by Time Of Flight), single-cell mass spectrometry, and advanced computational tools are often used to handle the complex data generated. These methods enable the identification and quantification of thousands of proteins from a single cell, providing a comprehensive view of the cellular proteome.
