Single cell sequencing involves isolating individual cells from a sample, amplifying their genetic material, and then sequencing it. The process generally includes the following steps:
Cell Isolation: Techniques such as fluorescence-activated cell sorting (FACS) or microfluidics are used to isolate single cells. Library Preparation: The genetic material from each cell is amplified and prepared for sequencing. Sequencing: High-throughput sequencing technologies are employed to read the amplified genetic material. Data Analysis: Bioinformatics tools are used to analyze the sequencing data, identifying genetic variants, gene expression patterns, and other features.
