How is Density Gradient Centrifugation Used to Isolate Exosomes?
Exosomes are small vesicles secreted by cells that play a significant role in cell-cell communication and tumor progression. Density gradient centrifugation is one of the most effective methods to isolate exosomes. Researchers use a sucrose gradient to separate exosomes from other cellular debris and proteins, allowing them to study the exosomal RNA, DNA, and proteins.
