How is Differential Gene Expression Analysis Performed?
The analysis typically involves several steps:
1. Sample Collection: Collecting tissue or cell samples from cancerous and normal tissues. 2. RNA Extraction and Sequencing: Extracting RNA from the samples and sequencing it using technologies like RNA-Seq. 3. Data Preprocessing: Quality control, alignment of sequences to a reference genome, and quantification of gene expression levels. 4. Statistical Analysis: Using statistical methods to identify genes with significant differences in expression between the groups. 5. Validation: Confirming findings through additional experiments such as qPCR or Western blotting.
