Several methods are used to detect mycoplasma contamination in cell cultures:
Polymerase Chain Reaction (PCR): This highly sensitive technique amplifies mycoplasma DNA, allowing for early detection of contamination. Enzyme-Linked Immunosorbent Assay (ELISA): This method uses antibodies to detect mycoplasma antigens in cell cultures. Culture Method: Mycoplasma can be cultured on specific media, though this method is time-consuming and less sensitive. Direct Staining: Fluorescent dyes like DAPI can stain mycoplasma DNA, which is then observed under a microscope.
