Pathway analysis typically involves the following steps:
Data Collection: Gather omics data from cancer tissues or cell lines. Data Normalization: Normalize the data to remove technical variations and biases. Identification of Differentially Expressed Genes/Proteins: Use statistical methods to identify genes or proteins that are differentially expressed in cancer versus normal samples. Pathway Enrichment Analysis: Use bioinformatics tools like Gene Set Enrichment Analysis (GSEA) to identify pathways that are significantly enriched in the differentially expressed genes or proteins. Network Analysis: Construct interaction networks to visualize and analyze the relationships between different molecules and pathways.
