Proteomic studies in cancer typically involve several key steps:
Sample Preparation: Tissues or cells are collected from cancer patients or experimental models. Protein Extraction and Digestion: Proteins are extracted from the samples and digested into peptides using enzymes like trypsin. Mass Spectrometry (MS): The peptides are analyzed using mass spectrometry to determine their mass-to-charge ratios, generating spectra that can be used to identify and quantify the proteins. Data Analysis: The MS data is processed using bioinformatics tools to identify proteins and quantify their abundance. Comparative analysis between cancerous and normal samples can reveal differentially expressed proteins.
