The H&E staining process involves several steps. First, tissue samples are fixed, usually in formalin, to preserve cellular structures. They are then embedded in paraffin wax to create a solid block that can be sliced into thin sections. These sections are placed on glass slides and dipped in hematoxylin, which stains the nuclei. After rinsing, the slides are counterstained with eosin, which highlights the cytoplasm and other structures. The stained slides are then dehydrated, cleared, and mounted for microscopic examination.
