The most common methods for collecting transcriptomic data include RNA sequencing (RNA-Seq) and microarrays. RNA-Seq uses next-generation sequencing technologies to provide a comprehensive view of the transcriptome, allowing for the quantification of gene expression levels and the identification of novel transcripts and splice variants. Microarrays, although less common now, involve hybridizing RNA to a grid of DNA probes and measuring fluorescence to infer gene expression levels.
