What Are Some Key Techniques Used in Cancer Research?
Polymerase Chain Reaction (PCR): This technique amplifies segments of DNA, allowing researchers to study genetic mutations associated with cancer. Next-Generation Sequencing (NGS): An advanced method for sequencing DNA or RNA to identify genetic variations linked to cancer susceptibility and resistance to treatment. CRISPR-Cas9: A genome-editing tool that allows precise modifications of DNA, used to create models for studying cancer genes. Western Blotting: Used to detect specific proteins in a sample, helping in the study of protein expression and modifications in cancer cells. Immunohistochemistry (IHC): A technique for visualizing the presence and location of proteins in tissue sections, critical for cancer diagnosis and prognosis.