What are the Challenges Associated with Fluorescent Antibodies?
Despite their benefits, fluorescent antibodies also have limitations: 1. Non-specific Binding: This can lead to background noise and false-positive results. 2. Photobleaching: Prolonged exposure to light can degrade the fluorescent signal, complicating long-term studies. 3. Quantification: Measuring fluorescence intensity accurately can be challenging and may require sophisticated imaging techniques. 4. Cost: High-quality fluorescent antibodies and imaging equipment can be expensive.
