Despite its potential, methylation profiling faces several challenges:
Technical variability: Different methods for methylation profiling can yield varying results, making it difficult to compare findings across studies. Heterogeneity: Tumors are often heterogeneous, with different regions showing distinct methylation patterns. This heterogeneity can complicate the interpretation of methylation data. Data complexity: Methylation profiling generates vast amounts of data, requiring sophisticated bioinformatics tools to analyze and interpret. Clinical integration: Incorporating methylation profiling into routine clinical practice requires overcoming regulatory, logistical, and cost barriers.
