What are the limitations of cell viability assays?
While cell viability assays are invaluable, they have certain limitations:
- Non-specificity: Some assays may not distinguish between cytostatic (growth-inhibiting) and cytotoxic (cell-killing) effects. - Interference: Compounds under investigation may interfere with assay reagents or readouts. - Cell Line Variability: Results can vary significantly between different cell lines, making it essential to validate findings across multiple models.
