What Are the Limitations of Gel Electrophoresis in Cancer Research?
Despite its widespread use, gel electrophoresis has certain limitations:
1. Resolution: The technique may not always provide high-resolution separation for very large or very small molecules. 2. Quantification: While it can separate molecules, quantifying them precisely often requires additional techniques. 3. Sensitivity: It may not be sensitive enough to detect very low-abundance molecules, which can be critical in early cancer detection. 4. Subjectivity: Interpretation of gel patterns can sometimes be subjective and require experienced personnel.
