Despite its advantages, NIRF has some limitations:
Limited Depth: While NIRF penetrates deeper than visible light, its depth of penetration is still limited compared to other imaging modalities like MRI or CT scans. Potential Toxicity: The long-term safety of fluorescent dyes used in NIRF is still under investigation, and their potential toxicity remains a concern. Technical Challenges: The accuracy of NIRF imaging can be affected by tissue autofluorescence and the uneven distribution of fluorescent probes.
