What are the Limitations of Real-Time PCR in Cancer?
Despite its many advantages, real-time PCR has some limitations. It requires high-quality DNA or RNA samples, and any contamination can affect the accuracy of the results. Additionally, it may not detect all genetic variations, particularly those that are rare or occur in regions not covered by the primers. Moreover, real-time PCR is generally limited to the analysis of known genetic targets, making it less suitable for discovering new mutations or biomarkers.
