methylation specific pcr

What Are the Steps in MSP?

1. Bisulfite Treatment: DNA is treated with sodium bisulfite, converting unmethylated cytosines to uracil.
2. PCR Amplification: Two sets of primers are used—one specific for methylated DNA and the other for unmethylated DNA.
3. Gel Electrophoresis: The PCR products are separated on an agarose gel to determine the presence of methylated or unmethylated DNA.

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