What are the Steps Involved in Microarray Analysis?
The process of microarray analysis generally involves several key steps:
Sample Collection: Obtaining tissue or cell samples from cancer patients and control subjects. RNA Extraction: Isolating total RNA from the collected samples. cDNA Synthesis: Converting RNA into complementary DNA (cDNA). Labeling: Labeling the cDNA with fluorescent dyes. Hybridization: Incubating the labeled cDNA with the microarray chip, allowing it to bind to complementary probes on the array. Scanning: Using a scanner to measure the fluorescence intensity of each spot on the array, which corresponds to the expression level of the gene. Data Analysis: Analyzing the scanned data to identify differentially expressed genes and pathways.
