What are the Steps Involved in the FISH Procedure?
The FISH procedure involves several steps:
Sample Preparation: The biological sample, which could be tissue, blood, or bone marrow, is prepared and fixed onto a microscope slide. Probe Labeling: Fluorescent probes specific to the DNA sequences of interest are synthesized and labeled with fluorescent dyes. Hybridization: The labeled probes are applied to the sample, where they hybridize with their complementary DNA sequences. Washing: Excess probes are washed away to reduce non-specific binding. Visualization: The hybridized probes are visualized using a fluorescence microscope, allowing for the detection of specific genetic abnormalities.
