Despite the potential, biomarker discovery faces several challenges: - Heterogeneity of Cancer: The genetic and molecular diversity in tumors makes it difficult to identify universal biomarkers. - Sample Quality and Variability: Differences in sample collection, processing, and storage can affect biomarker detection. - False Positives/Negatives: Ensuring high specificity and sensitivity to avoid incorrect diagnosis or missed detections is challenging. - Regulatory Hurdles: Gaining regulatory approval for new biomarkers involves extensive validation and can be time-consuming.
