What Challenges Exist in Fusion Gene Identification?
Identifying fusion genes is a complex task with several challenges:
Complexity of Cancer Genomes: Cancer genomes often have multiple mutations and structural variations, making it difficult to pinpoint specific fusion genes. Low Abundance: Some fusion genes are expressed at low levels, requiring highly sensitive techniques for detection. False Positives: High-throughput methods like NGS can produce false positives, necessitating additional validation steps.
