Several methods are employed to validate hits in cancer research:
Biochemical assays: These assays help confirm that the compound interacts with the target protein in a physiologically relevant manner. Cell-based assays: These methods test the compound's effect on cancer cell lines to ensure it induces the desired biological response. Genetic validation: Techniques like CRISPR and RNA interference (RNAi) are used to modulate the target gene and observe the compound's effect, confirming its mechanism of action. In vivo studies: Animal models are used to evaluate the compound's efficacy and safety in a living organism, providing insights into its potential clinical relevance.
