Several technologies have been developed to facilitate 3D cell culture, including scaffold-based methods, hydrogels, and bioreactors. Scaffold-based methods use materials like collagen or synthetic polymers to provide a structure for cells to grow on. Hydrogels offer a gel-like environment that can encapsulate cells, allowing them to grow in a more natural state. Bioreactors provide dynamic conditions, such as fluid flow and mechanical forces, to further mimic the in vivo environment. Each of these technologies has its own advantages and can be chosen based on the specific requirements of the study.
