Several methods are used to create 3D cell cultures, each with its own set of advantages and limitations:
- Spheroid Cultures: Cells are allowed to aggregate into spherical clusters, known as spheroids. These are simple to create and are widely used for studying cancer cell behavior and drug responses.
- Organoids: These are miniaturized, simplified versions of organs created from stem cells. Organoids provide a more complex and physiologically relevant model than spheroids.
- Hydrogels and Scaffolds: Cells are embedded in hydrogels or grown on scaffolds made of materials like collagen or synthetic polymers. These structures provide a more defined architecture for cell growth and interaction.
