Determining immune subtypes involves various techniques, including:
- Immunohistochemistry (IHC): Used to visualize the presence and location of immune cells within the tumor tissue. - Flow Cytometry: Helps in quantifying different immune cell populations. - Gene Expression Profiling: Assesses the expression of immune-related genes, providing a comprehensive view of the immune landscape. - Multiplex Immunofluorescence: Allows simultaneous detection of multiple immune markers in a single tissue section.