The detection and quantification of ncRNAs in cancer research and diagnostics involve several techniques:
1. qRT-PCR: Quantitative reverse transcription PCR is commonly used for measuring miRNA and lncRNA levels. 2. RNA Sequencing: High-throughput sequencing provides a comprehensive profile of ncRNA expression. 3. Microarrays: These are used for profiling the expression levels of multiple ncRNAs simultaneously.