Point mutations can be detected using various molecular biology techniques. Some of the most commonly used methods include:
- Polymerase chain reaction (PCR): This technique can amplify specific DNA sequences to detect mutations. - Sequencing technologies: Methods such as Sanger sequencing and next-generation sequencing (NGS) allow for the precise identification of point mutations. - Allele-specific oligonucleotide (ASO) hybridization: This technique uses probes that specifically bind to mutant sequences, enabling the detection of point mutations.
