Proteomic profiling typically involves several key techniques:
1. Mass Spectrometry (MS): This is the most commonly used method for protein identification and quantification. MS can analyze complex protein mixtures and provide detailed information on protein structure and modifications. 2. Two-Dimensional Gel Electrophoresis (2-DE): This technique separates proteins based on their isoelectric point and molecular weight, allowing for the visualization of protein expression patterns. 3. Liquid Chromatography (LC): Often coupled with MS, LC is used to separate proteins or peptides in a mixture before identification. 4. Western Blotting: This method is used to detect specific proteins in a sample using antibody binding.
