Protein microarrays function by immobilizing a collection of proteins on a solid surface, such as a glass slide. The surface is then exposed to various biological samples, such as serum or cell lysates, which contain proteins of interest. Interactions between the immobilized proteins and the sample proteins are detected using specific probes, often labeled with fluorescent or chemiluminescent tags. The resulting signals are analyzed to determine the presence and quantity of specific protein interactions.
