The process of pyrosequencing involves several steps: 1. Sample Preparation: DNA is extracted from cancer cells. 2. PCR Amplification: The target DNA region is amplified using PCR. 3. Sequencing Reaction: The amplified DNA is subjected to a sequencing reaction where nucleotides are added one by one. 4. Detection: Pyrophosphate release during nucleotide incorporation is detected, producing a light signal that is proportional to the number of nucleotides added.
