Establishing a cancer cell culture involves several steps:
Tissue Collection: Obtaining tumor samples from patients or animal models. Cell Isolation: Separating cancer cells from the surrounding tissues using enzymatic or mechanical methods. Culturing Cells: Growing isolated cells in a nutrient-rich medium under controlled conditions of temperature, humidity, and gas composition. Monitoring and Maintenance: Regularly checking cell growth and health, passaging cells to prevent overgrowth and contamination.