culture method

How is a Cancer Cell Culture Established?

Establishing a cancer cell culture involves several steps:
Tissue Collection: Obtaining tumor samples from patients or animal models.
Cell Isolation: Separating cancer cells from the surrounding tissues using enzymatic or mechanical methods.
Culturing Cells: Growing isolated cells in a nutrient-rich medium under controlled conditions of temperature, humidity, and gas composition.
Monitoring and Maintenance: Regularly checking cell growth and health, passaging cells to prevent overgrowth and contamination.

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