Gene amplification can be detected using a variety of techniques, including: - Fluorescence in situ hybridization (FISH): This technique uses fluorescent probes to identify specific DNA sequences. - Quantitative PCR (qPCR): Measures the amount of DNA to determine the number of gene copies. - Comparative Genomic Hybridization (CGH): Compares the DNA of cancer cells to normal cells to identify amplified regions. - Next-Generation Sequencing (NGS): Provides a comprehensive view of the genome, allowing for detailed detection of amplification events.
