Fixation: Tissue samples are preserved using a fixative, typically formalin, to prevent degradation and maintain cellular structures. Embedding: Fixed tissues are embedded in a medium, usually paraffin wax, to provide support for thin sectioning. Sectioning: The embedded tissue is cut into thin slices, usually 4-5 micrometers thick, using a microtome. Staining: The tissue sections are subjected to the appropriate histochemical stains. This may involve multiple steps, including washing, incubation with stains, and counterstaining. Mounting: Stained sections are mounted on glass slides and covered with a coverslip for examination under a microscope.
