qPCR is utilized in cancer diagnosis by detecting specific genetic alterations associated with certain cancers. For instance:
BRCA1/2 Mutations: qPCR can identify BRCA1/2 mutations linked to breast and ovarian cancers. BCR-ABL Fusion Gene: In chronic myeloid leukemia (CML), qPCR detects the BCR-ABL fusion gene, aiding in diagnosis and monitoring. EGFR Mutations: qPCR can detect EGFR mutations in non-small cell lung cancer, guiding targeted therapy.
