RT-PCR is extensively used in cancer research for multiple purposes:
Gene Expression Profiling: It helps in quantifying the expression levels of genes associated with cancer, allowing researchers to identify biomarkers and cancer-specific signatures. Detection of Fusion Genes: Certain cancers are characterized by specific fusion genes, like the BCR-ABL fusion in chronic myeloid leukemia (CML). RT-PCR can detect these fusion genes, aiding in diagnosis. Monitoring Minimal Residual Disease: In patients undergoing cancer treatment, RT-PCR can be used to detect minimal residual disease (MRD) by identifying low levels of cancer-specific RNA. Validation of Microarray Data: RT-PCR is often used to validate results obtained from microarray studies, ensuring the accuracy of gene expression data.
