RT-PCR offers several advantages that make it a preferred method in cancer research:
Sensitivity: RT-PCR is highly sensitive, capable of detecting low levels of RNA, which is crucial for identifying early-stage cancers or residual disease. Specificity: The technique is highly specific, allowing for precise amplification of target sequences. Quantitative Data: Real-time RT-PCR (qRT-PCR) provides quantitative data, enabling researchers to measure the exact expression levels of genes. Speed: Compared to other methods like Northern blotting, RT-PCR is faster, delivering results in a shorter time frame.
