Data Quality Control: Ensuring the raw data is of high quality by removing low-quality reads and contaminants. Alignment: Mapping the sequencing reads to a reference genome to identify variations. Variant Calling: Detecting genetic mutations, including single nucleotide variants (SNVs), insertions, deletions, and structural variations. Annotation: Linking identified variants to known genes, pathways, and diseases. Interpretation: Understanding the biological significance of the variants and their implications for cancer development and treatment.
