What are Some Common Genomic Techniques Used in Cancer?
Several genomic techniques are widely used in cancer research, including:
Next-Generation Sequencing (NGS): This technique allows for the rapid sequencing of entire genomes or specific regions, providing detailed insights into genetic mutations. Whole Exome Sequencing (WES): Focuses on sequencing the exonic regions of the genome, which are the parts that code for proteins. RNA Sequencing (RNA-Seq): Used to analyze the transcriptome, giving information on gene expression levels and discovering new RNA species. Comparative Genomic Hybridization (CGH): A technique for detecting genomic copy number changes, such as amplifications and deletions. CRISPR-Cas9: A genome-editing tool that allows for specific alterations in the DNA sequence, aiding in functional studies of cancer genes. Single-cell Sequencing: Enables the analysis of genetic material at the single-cell level, providing insights into tumor heterogeneity.
