What Are Some Limitations of the Boyden Chamber Assay?
While the Boyden chamber assay is highly informative, it has some limitations. One limitation is that it does not replicate the complex tumor microenvironment found in vivo. Additionally, the assay primarily measures 2D migration, which may not fully capture the 3D invasion characteristics of cancer cells. Despite these limitations, the assay remains a cornerstone in the study of cancer cell motility.
