What are the Challenges and Limitations of Mutect2?
Despite its advantages, Mutect2 has some challenges and limitations:
Complexity: Requires expertise in bioinformatics for optimal use and interpretation of results. Computational Resources: Demands significant computational power and storage, especially for large datasets. False Negatives: Although designed to minimize false positives, there is a risk of missing true variants, especially in low-quality or low-coverage regions.
