What Are the Challenges of CRISPR Screening in Cancer?
Despite its advantages, there are challenges associated with CRISPR screening:
Off-Target Effects: Although CRISPR is precise, unintended genetic modifications can still occur, potentially confounding results. Delivery: Efficiently delivering CRISPR components into cells, especially in vivo, remains a significant hurdle. Complexity of Cancer: Cancer's heterogeneity and complexity mean that results from CRISPR screens need careful interpretation and validation.
