Despite its advantages, NGS faces several challenges: - Data Complexity: The massive amount of data requires sophisticated bioinformatics tools for analysis and interpretation. - Cost: While costs have decreased, NGS can still be expensive, especially for whole-genome sequencing. - Ethical and Privacy Concerns: Handling and storing genetic data raise issues related to patient privacy and consent. - Clinical Validation: Ensuring that identified genetic alterations are clinically relevant and actionable remains a significant hurdle.
