What are the Common Assays Used in In Vitro Screening?
Several assays are used to assess the effects of compounds on cancer cells. Some of the most common ones include:
MTT assay: Measures cell metabolic activity as an indicator of cell viability. Annexin V/PI staining: Detects apoptosis by measuring phosphatidylserine exposure and membrane integrity. BrdU assay: Monitors cell proliferation by measuring DNA synthesis. Caspase activity assay: Evaluates apoptosis by detecting caspase enzyme activity. Western blotting: Analyzes protein expression levels and modifications.
