Flexibility: Limma can handle various types of experimental designs, including complex ones with multiple factors and interactions. Robustness: The empirical Bayes moderation helps in providing stable estimates even with small sample sizes. Comprehensive Documentation: Limma comes with extensive documentation and tutorials, making it accessible to both novice and experienced users. Integration with R: Limma is implemented in the R programming language, allowing seamless integration with other bioinformatics tools and packages.